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Assessing the genetic structure of Oryza glumaepatula populations with isozyme markers BABT
Veasey,Elizabeth Ann; Cardin,Daruska; Silva,Rainério Meireles; Bressan,Eduardo de Andrade; Vencovsky,Roland.
To assess the genetic diversity and genetic structure parameters, nine populations of Oryza glumaepatula from the Amazon biome, four from the Pantanal biome, and one collected at Rio Xingu, Mato Grosso, totaling 14 populations and 333 individuals were studied with isozyme markers. Six loci were evaluated showing a moderate allozyme variability (A = 1.21, P = 20.7%, Ho = 0.005, He = 0.060). The populations from the Pantanal biome showed higher diversity levels than the Amazon biome. High genetic differentiation among the populations, expected for self-fertilizing species, was observed (F ST=0.763), with lower differentiation found among the Pantanal populations (F ST=0.501). The average apparent outcrossing rate was higher for the Pantanal populations (t a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; Isozymes; Oryza glumaepatula; Outcrossing rate; Populations.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500001
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CTAB methods for DNA extraction of sweetpotato for microsatellite analysis Scientia Agricola
Borges,Aline; Rosa,Mariana Silva; Recchia,Gustavo Henrique; Queiroz-Silva,Jurema Rosa de; Bressan,Eduardo de Andrade; Veasey,Elizabeth Ann.
Microsatellite markers have proved to be useful in genetic diversity assessments of sweetpotato (Ipomoea batatas) but practical DNA extraction methods to ensure good quality and quantity DNA for these studies are yet to be established. This study compares the efficiency of three modified methodologies for DNA extraction of six sweetpotato landraces using the CTAB extraction buffer in regard to quantity and purity of DNA quantification and microsatellite band patterns. All methodologies yielded satisfactory results, but the method based in leaf tissue macerated in liquid nitrogen was deemed more adequate because of its simplicity and lower cost. However, the method based in dry leaf tissue was considered more advantageous, first because elicits...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ipomoea batatas; SSR; DNA isolation; Landraces; Protocol.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000400015
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Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers Genet. Mol. Biol.
Veasey,Elizabeth Ann; Borges,Aline; Rosa,Mariana Silva; Queiroz-Silva,Jurema R.; Bressan,Eduardo de Andrade; Peroni,Nivaldo.
We used simple sequence repeat (SSR) markers to investigate the genetic diversity of 78 sweet potato (Ipomoea batatas) accessions (58 landraces and 20 putative clones) from traditional agricultural households from 19 local communities in the Vale do Ribeira, São Paulo, Brazil. Eight SSR loci were assessed using 6% (w/v) polyacrylamide gels stained with silver nitrate and the accessions genotyped considering the presence or absence of bands. The results were subjected to analysis of molecular variance (AMOVA), and cluster and principal coordinate analyses. Spatial structure was assessed using Mantel's test to compare genetic and geographic distances. Each primer pair generated between three and ten clearly scorable polymorphic fragments. Cluster analyses...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variability; Landraces; SSR; Traditional agriculture; Vale do Ribeira.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400020
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Genetic diversity of American wild rice species Scientia Agricola
Veasey,Elizabeth Ann; Bressan,Eduardo de Andrade; Zucchi,Maria Imaculada; Vencovsky,Roland; Cardim,Daruska Cavalcante; Silva,Rainério Meireles da.
Studies on genetic diversity and genetic structure of natural populations are important in order to define strategies for in situ and ex situ conservation actions and for plant pre-breeding programs. Aiming to assess the genetic diversity and genetic structure of three wild American Oryza species with isozyme markers, 14 populations of the diploid O. glumaepatula (AglAgl), 11 populations of the tetraploid O. grandiglumis (CCDD) and five populations of the also tetraploid O. latifolia (CCDD) were studied. They were all originated from Rio Paraguay hydrographic basin and the Amazon. Four enzymes were used and they gave 40 polymorphic bands. The most polymorphic species was O. glumaepatula, followed by O. latifolia and O. grandiglumis. A cluster analysis with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oryza glumaepatula; O. latifolia; O. grandiglumis; Amazon; Genetic structure.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400008
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Morphological variation and isozyme diversity in Dioscorea alata L. landraces from Vale do Ribeira, Brazil Scientia Agricola
Bressan,Eduardo de Andrade; Briner Neto,Thiago; Zucchi,Maria Imaculada; Rabello,Ronaldo José; Veasey,Elizabeth Ann.
Traditional growers of the Vale do Ribeira, São Paulo State, grow and make use of several D. alata landraces. This study assessed the genetic diversity of 16 landraces, using isozymatic and morphological markers and comparing them with 19 commercial varieties of D. alata. Their distribution in different levels of organization such as households and communities of the Vale do Ribeira was evaluated. Isozymatic analyses were performed with polyacrylamide (six systems) and starch gels (one system), while the morphological analyses were carried out with 24 traits. Due to the polyploid nature of this species, the isozymatic bands were scored as binary data. Morphological traits were also scored as binary data. Principal coordinates and cluster analyses were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AMOVA; Traditional agriculture; Genetic diversity; Water yam.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400016
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Phenology and morphological diversity of sweet potato (Ipomoea batatas) landraces of the Vale do Ribeira Scientia Agricola
Veasey,Elizabeth Ann; Silva,Jurema Rosa de Queiroz; Rosa,Mariana Silva; Borges,Aline; Bressan,Eduardo de Andrade; Peroni,Nivaldo.
The phenotypic diversity of sweet potato (Ipomoea batatas) landraces was assessed using morphological traits, verifying how this diversity is distributed among the households and settlements of the Vale do Ribeira, Brazil. A total of 74 accessions, involving 53 landraces, collected from 30 households distributed among 18 settlements that practice traditional agriculture in the municipalities of Iguape, Ilha Comprida, and Cananeia, as well as four commercial varieties acquired in markets of Iguape and Piracicaba, were evaluated under an ex situ experimental condition in Piracicaba, SP, Brazil. Nine phenological and floral descriptors, nine morphological vegetative aerial descriptors and five storage root traits were recorded. The 14 aerial vegetative and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Local varieties; Morphology; Phenotypic diversity; Traditional agriculture; Variability.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000400013
Registros recuperados: 6
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